A quantitative fluorescence-based steady-state assay of DNA polymerase
نویسندگان
چکیده
منابع مشابه
A quantitative fluorescence‐based steady‐state assay of DNA polymerase
Fluorescent dyes that bind DNA have been demonstrated as a useful alternative to radionucleotides for the quantification of DNA and the in vitro measurement of the activity of DNA polymerases and nucleases. However, this approach is generally used in a semi-quantitative way to determine relative rates of reaction. In this report, we demonstrate a method for the simultaneous quantification of DN...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2014
ISSN: 1742-464X
DOI: 10.1111/febs.12760